Uptake of Methotrexate Linked to Polyclonal and Monoclonal Antimelanoma Antibodies by a Human Melanoma Cell Line
Identifieur interne : 003452 ( Main/Exploration ); précédent : 003451; suivant : 003453Uptake of Methotrexate Linked to Polyclonal and Monoclonal Antimelanoma Antibodies by a Human Melanoma Cell Line
Auteurs : Patrick Uadia ; A. Huntley Blair ; Tarunendu Ghose ; Soldano FerroneSource :
- Journal of the National Cancer Institute [ 0027-8874 ] ; 1985.
Abstract
[3H] Methotrexate ([3H]MTX) was covalently linked to monoclonal antibody (MoAb) 225.28S against human melanoma, to a rabbit anti-human melanoma IgG absorbed either with human red blood cells (AHMGR) or with red blood cells and a variety of normal human tissues (AHMGR+T), or to normal rabbit IgG (NRG). Human melanoma M21 cells were incubated at 0° C or 37° C with 10 μM free MTX or 10 μM MTX linked to one of the above carriers. The order of net uptake of MTX during 6 hours was MTX-MoAb 225.28S > MTX-AHMGR > MTX-AHMGR+T > MTX-NRG ≥ MTX. This order of uptake by the three antibody conjugates corresponded to the amount of conjugate bound at equilibrium at 0°C and to the immunofluorescence titers. Binding sites for MoAb 225.28S were more efficient for internalization of MTX than were those for the two polyclonal antibody preparations. When M21 cells preloaded with MTX by incubation at a drug concentration of 1.0 or 10 μM were incubated in drug-free medium, the amount of cell-associated MTX rapidly declined to 1.8 pmol/mg protein, i.e., the level of intra-cellular dihydrofolate reductase (DHFR). However, when cells preloaded to a drug content of 112 pmol/mg protein by incubation with 10 μM MTX linked to AHMGR were transferred to conjugate-free medium, 65 pmol MTX/mg remained cell associated after 12 hours. The efflux was inhibited by chloroquine. Both the efflux medium and M21 cells after a 9.5-hour incubation period had MTX-containing catabolic fragments that inhibited DHFR.
Url:
DOI: 10.1093/jnci/74.1.29
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: 001265
- to stream Istex, to step Curation: 001265
- to stream Istex, to step Checkpoint: 002210
- to stream Main, to step Merge: 003526
- to stream Main, to step Curation: 003452
Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title>Uptake of Methotrexate Linked to Polyclonal and Monoclonal Antimelanoma Antibodies by a Human Melanoma Cell Line</title><author><name sortKey="Uadia, Patrick" sort="Uadia, Patrick" uniqKey="Uadia P" first="Patrick" last="Uadia">Patrick Uadia</name></author><author><name sortKey="Blair, A Huntley" sort="Blair, A Huntley" uniqKey="Blair A" first="A. Huntley" last="Blair">A. Huntley Blair</name></author><author><name sortKey="Ghose, Tarunendu" sort="Ghose, Tarunendu" uniqKey="Ghose T" first="Tarunendu" last="Ghose">Tarunendu Ghose</name></author><author><name sortKey="Ferrone, Soldano" sort="Ferrone, Soldano" uniqKey="Ferrone S" first="Soldano" last="Ferrone">Soldano Ferrone</name></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:F0DA9C853F75F46DEE9FA3E9B942A3A8B699D600</idno><date when="1985" year="1985">1985</date><idno type="doi">10.1093/jnci/74.1.29</idno><idno type="url">https://api.istex.fr/ark:/67375/HXZ-B6MP9LFV-1/fulltext.pdf</idno><idno type="wicri:Area/Istex/Corpus">001265</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">001265</idno><idno type="wicri:Area/Istex/Curation">001265</idno><idno type="wicri:Area/Istex/Checkpoint">002210</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">002210</idno><idno type="wicri:doubleKey">0027-8874:1985:Uadia P:uptake:of:methotrexate</idno><idno type="wicri:Area/Main/Merge">003526</idno><idno type="wicri:Area/Main/Curation">003452</idno><idno type="wicri:Area/Main/Exploration">003452</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">Uptake of Methotrexate Linked to Polyclonal and Monoclonal Antimelanoma Antibodies by a Human Melanoma Cell Line<ref type="fn" target="#FN2">2</ref></title><author><name sortKey="Uadia, Patrick" sort="Uadia, Patrick" uniqKey="Uadia P" first="Patrick" last="Uadia">Patrick Uadia</name></author><author><name sortKey="Blair, A Huntley" sort="Blair, A Huntley" uniqKey="Blair A" first="A. Huntley" last="Blair">A. Huntley Blair</name></author><author><name sortKey="Ghose, Tarunendu" sort="Ghose, Tarunendu" uniqKey="Ghose T" first="Tarunendu" last="Ghose">Tarunendu Ghose</name><affiliation><wicri:noCountry code="no comma">Address reprint requests to Dr. Ghose.</wicri:noCountry></affiliation></author><author><name sortKey="Ferrone, Soldano" sort="Ferrone, Soldano" uniqKey="Ferrone S" first="Soldano" last="Ferrone">Soldano Ferrone</name></author></analytic><monogr></monogr><series><title level="j" type="main">Journal of the National Cancer Institute</title><title level="j" type="abbrev">Journal of the National Cancer Institute</title><idno type="ISSN">0027-8874</idno><idno type="eISSN">1460-2105</idno><imprint><publisher>Oxford University Press</publisher><date when="1985-01">1985</date><biblScope unit="vol">74</biblScope><biblScope unit="issue">1</biblScope><biblScope unit="page" from="29">29</biblScope><biblScope unit="page" to="35">35</biblScope></imprint><idno type="ISSN">0027-8874</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0027-8874</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract">[3H] Methotrexate ([3H]MTX) was covalently linked to monoclonal antibody (MoAb) 225.28S against human melanoma, to a rabbit anti-human melanoma IgG absorbed either with human red blood cells (AHMGR) or with red blood cells and a variety of normal human tissues (AHMGR+T), or to normal rabbit IgG (NRG). Human melanoma M21 cells were incubated at 0° C or 37° C with 10 μM free MTX or 10 μM MTX linked to one of the above carriers. The order of net uptake of MTX during 6 hours was MTX-MoAb 225.28S > MTX-AHMGR > MTX-AHMGR+T > MTX-NRG ≥ MTX. This order of uptake by the three antibody conjugates corresponded to the amount of conjugate bound at equilibrium at 0°C and to the immunofluorescence titers. Binding sites for MoAb 225.28S were more efficient for internalization of MTX than were those for the two polyclonal antibody preparations. When M21 cells preloaded with MTX by incubation at a drug concentration of 1.0 or 10 μM were incubated in drug-free medium, the amount of cell-associated MTX rapidly declined to 1.8 pmol/mg protein, i.e., the level of intra-cellular dihydrofolate reductase (DHFR). However, when cells preloaded to a drug content of 112 pmol/mg protein by incubation with 10 μM MTX linked to AHMGR were transferred to conjugate-free medium, 65 pmol MTX/mg remained cell associated after 12 hours. The efflux was inhibited by chloroquine. Both the efflux medium and M21 cells after a 9.5-hour incubation period had MTX-containing catabolic fragments that inhibited DHFR.</div></front></TEI><affiliations><list></list><tree><noCountry><name sortKey="Blair, A Huntley" sort="Blair, A Huntley" uniqKey="Blair A" first="A. Huntley" last="Blair">A. Huntley Blair</name><name sortKey="Ferrone, Soldano" sort="Ferrone, Soldano" uniqKey="Ferrone S" first="Soldano" last="Ferrone">Soldano Ferrone</name><name sortKey="Ghose, Tarunendu" sort="Ghose, Tarunendu" uniqKey="Ghose T" first="Tarunendu" last="Ghose">Tarunendu Ghose</name><name sortKey="Uadia, Patrick" sort="Uadia, Patrick" uniqKey="Uadia P" first="Patrick" last="Uadia">Patrick Uadia</name></noCountry></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/ChloroquineV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 003452 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 003452 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= ChloroquineV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= ISTEX:F0DA9C853F75F46DEE9FA3E9B942A3A8B699D600
|texte= Uptake of Methotrexate Linked to Polyclonal and Monoclonal Antimelanoma Antibodies by a Human Melanoma Cell Line
}}
| This area was generated with Dilib version V0.6.33. |  |